Single cell RNA sequencing of AML initiating cells reveals RNA-based evolution during disease progression.

ABSTRACT: The prognosis of most patients with AML is poor due to frequent disease relapse. The cause of relapse is thought to be from the persistence of leukemia initiating cells (LIC's) following treatment. Here we assessed RNA based changes in LICs from matched patient diagnosis and relapse samples using single-cell RNA sequencing. Previous studies on AML progression have focused on genetic changes at the DNA mutation level mostly in bulk AML cells and demonstrated the existence of DNA clonal evolution. Here we identified in LICs that the phenomenon of RNA clonal evolution occurs during AML progression. Despite the presence of vast transcriptional heterogeneity at the single cell level, pathway analysis identified common signaling networks involving metabolism, apoptosis and chemokine signaling th

SUBMITTER: Stetson LC 

PROVIDER: S-EPMC8807029 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature