Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.

ABSTRACT: Mitophagy, the selective degradation of mitochondria by autophagy, affects defective mitochondria following damage or stress. At the onset of mitophagy, parkin ubiquitylates proteins on the mitochondrial outer membrane. While the role of parkin at the onset of mitophagy is well understood, less is known about its activity during later stages in the process. Here, we used HeLa cells expressing catalytically active or inactive parkin to perform temporal analysis of the proteome, ubiquitylome, and phosphoproteome during 18 h after induction of mitophagy by mitochondrial uncoupler carbonyl cyanide m-chlorophenyl hydrazine. Abundance profiles of proteins downregulated in parkin-dependent manner revealed a stepwise and "outside-in" directed degradation of mitochondrial subcompartments. While ubi

SUBMITTER: Zittlau KI 

PROVIDER: S-EPMC8808264 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature