Identification of potential modulators of osteosarcoma metastasis by high-throughput cellular screening of natural products.

ABSTRACT: A high-throughput screening assay was developed and applied to a large library of natural product extract samples, in order to identify compounds which preferentially inhibited the in vitro 2D growth of a highly metastatic osteosarcoma cell line (MG63.3) compared to a cognate parental cell line (MG63) with low metastatic potential. Evaluation of differentially active natural product extracts with bioassay-guided fractionation led to the identification of lovastatin (IC₅₀  = 11 µm) and the limonoid toosendanin (IC₅₀  = 26 nm). Other statins and limonoids were then tested, and cerivastatin was identified as a particularly potent (IC₅₀  < 0.1 µm) and selective agent. These compounds potently and selectively induced apoptosis in MG63.3 cells, but not MG63. Assa

SUBMITTER: Long SA 

PROVIDER: S-EPMC8808376 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature