Airway microbiome dynamics and relationship to ventilator-associated infection in intubated pediatric patients.

ABSTRACT:

Background

Little is known about the airway microbiome in intubated mechanically ventilated children. We sought to characterize the airway microbiome longitudinally and in association with clinical variables and possible ventilator-associated infection (VAI).

Methods

Serial tracheal aspirate samples were prospectively obtained from mechanically ventilated subjects under 3 years old from eight pediatric intensive care units in the United States from June 2017 to July 2018. Changes in the tracheal microbiome were analyzed by sequencing bacterial 16S ribosomal RNA gene relative to subject demographics, diagnoses, clinical parameters, outcomes, antibiotic treatment, and the Ventilator-Associated InfectioN (VAIN) score.

Results

A total of 221 samples from 58 patients were

SUBMITTER: Tarquinio KM 

PROVIDER: S-EPMC8809006 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature