Parallel analysis of transcription, integration, and sequence of single HIV-1 proviruses.

ABSTRACT: HIV-1-infected cells that persist despite antiretroviral therapy (ART) are frequently considered "transcriptionally silent," but active viral gene expression may occur in some cells, challenging the concept of viral latency. Applying an assay for profiling the transcriptional activity and the chromosomal locations of individual proviruses, we describe a global genomic and epigenetic map of transcriptionally active and silent proviral species and evaluate their longitudinal evolution in persons receiving suppressive ART. Using genome-wide epigenetic reference data, we show that proviral transcriptional activity is associated with activating epigenetic chromatin features in linear proximity of integration sites and in their inter- and intrachromosomal contact regions. Transcriptionally activ

SUBMITTER: Einkauf KB 

PROVIDER: S-EPMC8809251 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature