Project description:SARS-CoV and SARS-CoV-2, the causative agents of severe acute respiratory syndrome (SARS) and coronavirus disease 2019 (COVID-19), are genetically related positive-sense RNA viruses that may cause similar pathophysiology. Despite host could activate interferon responses upon coronaviral infection to suppress virus replication, both SARS-CoV and SARS-CoV-2 have evolved strategies to inhibit interferon response. Here, we constructed SARS-CoV and SARS-CoV-2 N proteins expressing cell lines (HEK293T-N and HEK293T-2N) and performed RNA sequencing analysis, showing that both SARS-CoV-2 and SARS-CoV N proteins could inhibit expression of early growth response gene 1 (EGR1) to suppress interferon response. Moreover, EGR1 could degrade N proteins of SARS-CoV and SARS-CoV-2 in a lysosome-dependent manner, and inhibit viral replication of SARS-CoV-2. Our findings revealed the important role of EGR1 in host innate immune response against SARS-CoV and SARS-CoV-2, which would contribute to understanding the pathogenesis of human coronaviruses and development of antiviral therapies. In addition, we demonstrated that both N proteins could upregulate expression of nervous development-related genes, which may be associated with the neurological symptoms of COVID-19 and SARS patients.

2023-08-20 | GSE189706 | GEO

Antibody response against Influenza and SARS-CoV-2 antigens

Project description:A protein microarray was used to measure antibody response in the plasma of mice immunized with mRNA vaccine formulations at d14 and d28 post prime immunizations (n = 53). The results demonstrate antigen- specific antibody response was elicited by a bivalent mRNA vaccine to combat Influenza and SARS-CoV-2 infections.

2025-04-23 | GSE295393 | GEO

Immunological features that associate with the strength of antibody responses to BNT162b2 mRNA vaccine against SARS-CoV-2.

Project description: Not available

| S-EPMC8872843 | biostudies-literature

Structural Basis of SARS-CoV-2 and SARS-CoV Antibody Interactions.

Project description: Not available

| S-EPMC7498231 | biostudies-literature