Analysis and verification of N⁶-methyladenosine-modified genes as novel biomarkers for clear cell renal cell carcinoma.

ABSTRACT: N⁶-methyladenosine (m⁶A) has been involved in diverse biological processes in cancer, but its function and clinical value in clear cell renal cell carcinoma (ccRCC) remain largely unknown. In this study, we found that 1453 m⁶A-modified differentially expressed genes (DEGs) of ccRCC were mainly enriched in cell cycle, PI3K-AKT, and p53 signaling pathways. Then we constructed a co-expression network of the 1453 m⁶A-modified DEGs and identified a most clinically relevant module, where NUF2, CDCA3, CKAP2L, KIF14, and ASPM were hub genes. NUF2, CDCA3, and KIF14 could combine with a major RNA m⁶A methyltransferase METTL14, serving as biomarkers for ccRCC. Real-time quantitative PCR assay confirmed that NUF2, CDCA3, and KIF14 were high

SUBMITTER: Yang Z 

PROVIDER: S-EPMC8810125 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature