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Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.

ABSTRACT: Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.

SUBMITTER: Kinoshita Y

PROVIDER: S-EPMC8810321 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.

Kinoshita Yuta Y Kakoi Hironaga H Ishige Taichiro T Yamanaka Takashi T Niwa Hidekazu H Uchida-Fujii Eri E Nukada Toshio T Ueno Takanori T

The Journal of veterinary medical science 20211202 1

Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 sam ...[more]

PMID: 34853198

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