CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

ABSTRACT:

Objective

Enhanced de novo lipogenesis (DNL) in hepatocytes is a major contributor to nonalcoholic fatty liver disease (NAFLD). Fatty acid translocase (FAT/CD36) is involved in the pathogenesis of NAFLD through facilitating free fatty acids uptake. Here, we explored the effects of CD36 on DNL and elucidated the underlying mechanisms.

Methods

We generated hepatocyte-specific CD36 knockout (CD36LKO) mice to study in vivo effects of CD36 on DNL under high-fat diet (HFD). Lipid deposition and DNL were analyzed in primary hepatocytes isolated from CD36LKO mice or HepG2 cells with CD36 overexpression. RNA sequence, co-immunoprecipitation, and proximity ligation assay were carried out to determine its role in regulating DNL.

Results

Hepatic CD36 expression was upregulated in

SUBMITTER: Zeng H 

PROVIDER: S-EPMC8810570 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature