Alpha adaptins show isoform-specific association with neurofibrillary tangles in Alzheimer's disease.

ABSTRACT:

Aims

The heterotetrameric assembly protein complex 2 (AP-2) is a central hub for clathrin-dependent endocytosis. The AP-2 α-adaptin subunit has two major isoforms, encoded by two separate genes: AP2A1 and AP2A2. Endocytosis has been implicated in the pathogenesis of neurodegenerative disease, and recent studies linked α-adaptins (gene variants, splicing defects and altered expression) with late-onset Alzheimer's disease (LOAD) risk. Here, we used multiple antibodies to investigate α-adaptin isoforms and their localization in human brains.

Methods

The specificities of 10 different α-adaptin antibodies were evaluated using immunoblots after human AP2A1 and AP2A2 plasmid transfection in cultured cells. Additional immunoblot analyses were then performed on protein homogenates fr

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC8810620 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature