Nicked Invader probes: multistranded and sequence-unrestricted recognition of double-stranded DNA.

ABSTRACT: Major efforts have been devoted to the development of constructs that enable sequence-specific recognition of double-stranded (ds) DNA, fueled by the promise for enabling tools for applications in molecular biology, diagnostics, and medicine. Towards this end, we have previously introduced Invader probes, i.e., short DNA duplexes with +1 interstrand zipper arrangements of intercalator-functionalized nucleotides. The individual strands of these labile probes display high affinity towards complementary DNA (cDNA), which drives sequence-unrestricted dsDNA-recognition. However, recognition of long targets is challenging due to the high stability of the corresponding probes. To address this, we recently introduced toehold Invader probes, i.e., Invader probes with 5'-single-strande

SUBMITTER: Adhikari SP 

PROVIDER: S-EPMC8810728 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature