Multiview motion tracking based on a cartesian robot to monitor Caenorhabditis elegans in standard Petri dishes.

ABSTRACT: Data from manual healthspan assays of the nematode Caenorhabditis elegans (C. elegans) can be complex to quantify. The first attempts to quantify motor performance were done manually, using the so-called thrashing or body bends assay. Some laboratories have automated these approaches using methods that help substantially to quantify these characteristic movements in small well plates. Even so, it is sometimes difficult to find differences in motor behaviour between strains, and/or between treated vs untreated worms. For this reason, we present here a new automated method that increases the resolution flexibility, in order to capture more movement details in large standard Petri dishes, in such way that those movements are less restricted. This method is based on a Cartesian robot, which en

SUBMITTER: Puchalt JC 

PROVIDER: S-EPMC8810772 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature