Correlation between targeted RNAseq signature of breast cancer CTCs and onset of bone-only metastases.

ABSTRACT:

Background

Bone is the most frequent site of metastases from breast cancer (BC), but no biomarkers are yet available to predict skeletal dissemination.

Methods

We attempted to identify a gene signature correlated with bone metastasis (BM) onset in circulating tumour cells (CTCs), isolated by a DEPArray-based protocol from 40 metastatic BC patients and grouped according to metastasis sites, namely "BM" (bone-only), "ES" (extra-skeletal) or BM + ES (bone + extra-skeletal).

Results

A 134-gene panel was first validated through targeted RNA sequencing (RNAseq) on sub-clones of the MDA-MB-231 BC cell line with variable organotropism, which successfully shaped their clustering. The panel was then applied to CTC groups and, in particular, the "BM" vs "ES" CTC comparison revea

SUBMITTER: Lovero D 

PROVIDER: S-EPMC8810805 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature