Development and validation of a circulating microRNA panel for the early detection of breast cancer.

ABSTRACT:

Background

Mammography is widely used for breast cancer screening but suffers from a high false-positive rate. Here, we perform the largest comprehensive, multi-center study to date involving diverse ethnic groups, for the identification of circulating miRNAs for breast cancer screening.

Methods

This study had a discovery phase (n = 289) and two validation phases (n = 374 and n = 379). Quantitative PCR profiling of 324 miRNAs was performed on serum samples from breast cancer (all stages) and healthy subjects to identify miRNA biomarkers. Two-fold cross-validation was used for building and optimising breast cancer-associated miRNA panels. An optimal panel was validated in cohorts with Caucasian and Asian samples. Diagnostic ability was evaluated using area under the curve (AU

SUBMITTER: Zou R 

PROVIDER: S-EPMC8810862 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature