Purification and characterization of human adipose-resident microvascular endothelial progenitor cells.

ABSTRACT: Human adipose tissue is a rich source of adipose-derived stem cells (ASCs) and vascular endothelial progenitor cells (EPCs). However, no standardized method has been established for the isolation and purification of adipose-derived EPCs (AEPCs). The aim of this study was to establish a method for the isolation and purification of AEPCs. The stromal vascular fraction (SVF) was extracted from human lipoaspirates, and the CD45^-CD31⁺ fraction of the SVF was collected by magnetic-activated cell sorting (MACS). The CD45^-CD31⁺ fraction was cultured for 4.5 days, followed by a second MACS separation to collect the CD31⁺ fraction. Purified AEPCs were expanded without being overwhelmed by proliferating ASCs, indicating that a high level (> 95%)

SUBMITTER: Saito N 

PROVIDER: S-EPMC8811023 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature