Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.

ABSTRACT:

Background

Auto-induction is a convenient way to produce recombinant proteins without inducer addition using lac operon-controlled Escherichia coli expression systems. Auto-induction can occur unintentionally using a complex culture medium prepared by mixing culture substrates. The differences in culture substrates sometimes lead to variations in the induction level.

Objectives

In this study, we investigated the feasibility of using glucose and lactose as boosters of auto-induction with a complex culture medium.

Methods

First, auto-induction levels were assessed by quantifying recombinant GFPuv expression under the control of the T7 lac promoter. Effectiveness of the additive-containing medium was examined using ovine angiotensinogen (tac promoter-based expression) an

SUBMITTER: Tahara N 

PROVIDER: S-EPMC8811614 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature