Multiscale imaging of therapeutic anti-PD-L1 antibody localization using molecularly defined imaging agents.

ABSTRACT:

Background

While immune checkpoint inhibitors such as anti-PD-L1 antibodies have revolutionized cancer treatment, only subgroups of patients show durable responses. Insight in the relation between clinical response, PD-L1 expression and intratumoral localization of PD-L1 therapeutics could improve patient stratification. Therefore, we present the modular synthesis of multimodal antibody-based imaging tools for multiscale imaging of PD-L1 to study intratumoral distribution of PD-L1 therapeutics.

Results

To introduce imaging modalities, a peptide containing a near-infrared dye (sulfo-Cy5), a chelator (DTPA), an azide, and a sortase-recognition motif was synthesized. This peptide and a non-fluorescent intermediate were used for site-specific functionalization of c-terminally so

SUBMITTER: Hagemans IM 

PROVIDER: S-EPMC8811974 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature