DNMT1 facilitates growth of breast cancer by inducing MEG3 hyper-methylation.

ABSTRACT:

Background

To understand the effect of DNMT1-mediated MEG3 promoter methylation on breast cancer progression.

Methods

Expression of DNMT1, MEG3 and miR-494-3p was assayed by qRT-PCR and western blot. Methylation-specific PCR was used to examine MEG3 promoter methylation level. ChIP, RNA binding protein immunoprecipitation assay and dual-luciferase reporter gene assay were applied to verify interaction between DNMT1 and MEG3, miR-494-3p and MEG3 and OTUD4. CCK-8, wound healing and Transwell assays were used to detect biological functions of breast cancer cells. Tumor growth was observed by tumor xenograft model.

Results

DNMT1 and miR-494-3p were highly expressed while MEG3 and OTUD4 were lowly expressed in breast cancer cells. Knockdown of DNMT1 inhibited progression o

SUBMITTER: Zhu X 

PROVIDER: S-EPMC8812010 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature