Quantitative proteomic analysis of GnRH agonist treated GBM cell line LN229 revealed regulatory proteins inhibiting cancer cell proliferation.

ABSTRACT:

Background

Gonadotropin-releasing hormone (GnRH) receptor, a rhodopsin-like G-protein coupled receptor (GPCR) family member involved in GnRH signaling, is reported to be expressed in several tumors including glioblastoma multiforme (GBM), one of the most malignant and aggressive forms of primary brain tumors. However, the molecular targets associated with GnRH receptor are not well studied in GBM or in other cancers. The present study aims at investigating the effect of GnRH agonist (Gosarelin acetate) on cell proliferation and associated signaling pathways in GBM cell line, LN229.

Methods

LN229 cells were treated with different concentrations of GnRH agonist (10^-10 M to 10^-5 M) and the effect on cell proliferation was analyzed by cell count method. Fur

SUBMITTER: Tripathi PH 

PROVIDER: S-EPMC8812247 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature