Disposable Autonomous Device for Swab-to-Result Diagnosis of Influenza.

ABSTRACT: A prototype of a self-contained, automated, disposable device for chemically amplified protein-based detection of influenza virus from nasal swab specimens was developed and evaluated in a clinical setting. The device required only simple specimen manipulation without any dedicated instrumentation or specialized training by the operator for interpretation. The device was based on a sandwich immunoassay for influenza virus nucleoprotein; it used an enzyme-labeled antibody and a chromogenic substrate to provide an amplified visible signal, in a two-dimensional paper network format. All reagents were stored within the device. Device performance was assessed at Seattle Children's Hospital; clinical staff collected nasal swab samples from 25 patients and then operated test devices on site to de

SUBMITTER: Huang S 

PROVIDER: S-EPMC8812334 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature