Adipogenic Differentiation Alters Properties of Vascularized Tissue-Engineered Skeletal Muscle.

ABSTRACT: Advances in the engineering of comprehensive skeletal muscle models in vitro will improve drug screening platforms and can lead to better therapeutic approaches for the treatment of skeletal muscle injuries. To this end, a vascularized tissue-engineered skeletal muscle (TE-SkM) model that includes adipocytes was developed to better emulate the intramuscular adipose tissue that is observed in skeletal muscles of patients with diseases such as diabetes. Muscle precursor cells cultured with and without microvessels derived from adipose tissue (microvascular fragments) were used to generate TE-SkM constructs, with and without a microvasculature, respectively. TE-SkM constructs were treated with adipogenic induction media to induce varying levels of adipogenesis. With a delayed addition

SUBMITTER: Acosta FM 

PROVIDER: S-EPMC8812504 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature