Mapping Replication Timing in Single Mammalian Cells.

ABSTRACT: Replication timing (RT) is the temporal order in which genomic DNA is replicated during S phase. Early and late replication correlate with transcriptionally active and inactive chromatin compartments, but mechanistic links between large-scale chromosome structure, transcription, and replication are still enigmatic. A proper RT program is necessary to maintain the global epigenome that defines cell identity, suggesting that RT is critical for epigenome integrity by facilitating the assembly of different types of chromatin at different times during S phase. RT is regulated during development and has been found to be altered in disease. Thus, RT can identify stable epigenetic differences distinguishing cell types, and can be used to help stratify patient outcomes and identify markers of disea

SUBMITTER: Bartlett DA 

PROVIDER: S-EPMC8812816 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature