Identifying cortical specific long noncoding RNAs modified by m⁶A RNA methylation in mouse brains.

ABSTRACT: Proper development of the mammalian cerebral cortex relies on precise gene expression regulation. Increasing evidence shows that cortical development is regulated by both mRNAs and long noncoding RNAs (lncRNAs), which also are modified by N⁶-methyladenosine (m⁶A). Patterns of m⁶A-methylation in lncRNAs in the developing cortex have not been uncovered. Here we reveal differentially expressed lncRNAs and report stage-specific m⁶A-methylation patterns in lncRNAs expressed in mouse embryonic (E) and postnatal (P) cortices using RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation (MeRIP) sequencing. Many lncRNAs show temporal differential expression, and display genic distribution in the genome. Interestingly, we detect temporal-spe

SUBMITTER: Nie Y 

PROVIDER: S-EPMC8813070 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature