MiR-204-5p Hampers Breast Cancer Malignancy and Affects the Cell Cycle by Targeting PRR11.

ABSTRACT:

Purpose

To unravel mechanisms of miR-204-5p in breast cancer (BC) cells.

Methods

miR-204-5p expression level in BC cell lines was measured by qRT-PCR. Putative binding sites of miR-204-5p on the 3'-untranslated region of PRR11 were predicted by the bioinformatics method and verified by the dual-luciferase method. Protein and mRNA levels of PRR11 in BC were determined by western blot and qRT-PCR. The association between two genes was analyzed by correlation analysis. Cancer cell functions were evaluated through CCK8, flow cytometry, and Transwell approaches.

Results

Significant downregulation of miR-204-5p was observed in BC tissue and cells. Cell functional experiments showed the inhibition of miR-204-5p on cell behaviors and cell cycle. PRR11 was the downstream targe

SUBMITTER: Su Q 

PROVIDER: S-EPMC8813226 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature