Screening of compounds to identify novel epigenetic regulatory factors that affect innate immune memory in macrophages.

ABSTRACT: Trained immunity and tolerance are part of the innate immune memory that allow innate immune cells to differentially respond to a second encounter with stimuli by enhancing or suppressing responses. In trained immunity, treatment of macrophages with β-glucan (BG) facilitates the production of proinflammatory cytokines upon lipopolysaccharide (LPS) stimulation. For the tolerance response, LPS stimulation leads to suppressed inflammatory responses during subsequent LPS exposure. Epigenetic reprogramming plays crucial roles in both phenomena, which are tightly associated with metabolic flux. In this study, we performed a screening of an epigenetics compound library that affects trained immunity or LPS tolerance in macrophages using TNFα as a readout. Among the 181 compounds tested, one compou

SUBMITTER: Benjaskulluecha S 

PROVIDER: S-EPMC8814160 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature