Fluoride-Controlled Riboswitch-Based Dampening of Gene Expression for Cloning Potent Promoters.

ABSTRACT: Bioreporter systems based on detectable enzyme activity, such as that of beta-galactosidase or luciferase, are key in novel bacterial promoter discovery and study. While these systems permit quantification of gene expression, their use is limited by the toxicity of the expressed reporter enzymes in a given host. Indeed, the most potent promoters may be overlooked if their activity causes a lethal overproduction of the reporter genes when screening for transcriptional activity of potential promoter sequences with the luxCDABE cassette. To overcome this limitation, a variation of the mini-CTX-lux plasmid has been designed which allows reduction of promoter activity via the addition of an adjacent fluoride riboswitch. The riboswitch adds a layer of regulation between the

SUBMITTER: Korniakova V 

PROVIDER: S-EPMC8814607 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature