Mechanism of Atg9 recruitment by Atg11 in the cytoplasm-to-vacuole targeting pathway.

ABSTRACT: Autophagy is a lysosomal degradation pathway for the removal of damaged and superfluous cytoplasmic material. This is achieved by the sequestration of this cargo material within double-membrane vesicles termed autophagosomes. Autophagosome formation is mediated by the conserved autophagy machinery. In selective autophagy, this machinery including the transmembrane protein Atg9 is recruited to specific cargo material via cargo receptors and the Atg11/FIP200 scaffold protein. The molecular details of the interaction between Atg11 and Atg9 are unclear, and it is still unknown how the recruitment of Atg9 is regulated. Here we employ NMR spectroscopy of the N-terminal disordered domain of Atg9 (Atg9-NTD) to map its interaction with Atg11 revealing that it involves two short peptides both contai

SUBMITTER: Coudevylle N 

PROVIDER: S-EPMC8814668 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature