A protocol for investigating lipidomic dysregulation and discovering lipid biomarkers from human serums.
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ABSTRACT:
SUBMITTER: Chen M
PROVIDER: S-EPMC8814823 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
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A protocol for investigating lipidomic dysregulation and discovering lipid biomarkers from human serums.
Chen Moran M Hao Yanhong Y Chen Suming S
STAR protocols 20220202 1
Lipids play important roles in various human diseases. Disease-associated lipid dysregulation and biomarkers could provide molecular clues for diagnosis, pathogenesis, and therapy. This protocol provides a step-by-step workflow to investigate lipid dysregulation and discover biomarkers in human serum samples by liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and machine learning analysis. The workflow includes project design, serum collection, sample preparation, data acquisitio ...[more]
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