Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples.

ABSTRACT: Gene expression analysis by RNA sequencing (RNA-seq) enables unique insights into clinical samples that can potentially lead to mechanistic understanding of the basis of various diseases as well as resistance and/or susceptibility mechanisms. However, FFPE tissues, which represent the most common method for preserving tissue morphology in clinical specimens, are not the best sources for gene expression profiling analysis. The RNA obtained from such samples is often degraded, fragmented, and chemically modified, which leads to suboptimal sequencing libraries. In turn, these generate poor quality sequence data that may not be reliable for gene expression analysis and mutation discovery. In order to make the most of FFPE samples and obtain the best possible data from low quality samples, it i

SUBMITTER: Levin Y 

PROVIDER: S-EPMC8815082 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature