N6-methyladenosine-dependent modification of circGARS acts as a new player that promotes SLE progression through the NF-κB/A20 axis.

ABSTRACT:

Background

Certain circRNAs could be used as biomarkers to determine the risk of development and/or severity of systemic lupus erythematosus, and their new function in the regulation of gene expression has motivated us to investigate their role in SLE METHODS: Experimental methods including qRT-PCR, RNA immunoprecipitation (RIP), pulldown, dual luciferase reporter assay, RNA interference and cell transfection, RNA fluorescence in situ hybridization, western blotting, and mass spectrometry were used to assessed circGARS (hsa_circRNA_0009000) for immune functions and defined mechanisms by which circGARS promotes the progression in SLE.

Results

Our results demonstrated that the levels of circGARS was remarkably upregulated in SLE and correlated with clinicopathological features

SUBMITTER: Zhao X 

PROVIDER: S-EPMC8815128 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature