Multiple distinct domains of human XIST are required to coordinate gene silencing and subsequent heterochromatin formation.

ABSTRACT:

Background

Mammalian dosage compensation is achieved by the inactivation of one X chromosome in XX individuals. In eutheria this process is initiated early in development by the long non-coding RNA XIST. Studies of the initiation of silencing by XIST have focussed on mouse models, so the domains of XIST required to induce silencing in humans, and their relationship with domains required to establish heterochromatin remain to be determined.

Methods

We have previously established an inducible XIST cDNA in somatic cells and shown it can induce silencing and recruit heterochromatic features. We now assess a series of deletions across the transgene for the ability to induce silencing and integrate these results with time-course and chromatin-remodelling inhibitor treatments to fo

SUBMITTER: Dixon-McDougall T 

PROVIDER: S-EPMC8815261 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature