Multiscale Assay of Unlabeled Neurite Dynamics Using Phase Imaging with Computational Specificity.

ABSTRACT: Primary neuronal cultures have been widely used to study neuronal morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning and memory. However, the unique behavioral properties of neurons make them challenging to study, with phenotypic differences expressed as subtle changes in neuronal arborization rather than easy-to-assay features such as cell count. The need to analyze morphology, growth, and intracellular transport has motivated the development of increasingly sophisticated microscopes and image analysis techniques. Due to its high-contrast, high-specificity output, many assays rely on confocal fluorescence microscopy, genetic methods, or antibody staining techniques. These approaches often limit the ability to measur

SUBMITTER: Kandel ME 

PROVIDER: S-EPMC8815662 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature