Generation of NKX2.5^GFP Reporter Human iPSCs and Differentiation Into Functional Cardiac Fibroblasts.

ABSTRACT: Direct cardiac reprogramming has emerged as an interesting approach for the treatment and regeneration of damaged hearts through the direct conversion of fibroblasts into cardiomyocytes or cardiovascular progenitors. However, in studies with human cells, the lack of reporter fibroblasts has hindered the screening of factors and consequently, the development of robust direct cardiac reprogramming protocols.In this study, we have generated functional human NKX2.5^GFP reporter cardiac fibroblasts. We first established a new NKX2.5^GFP reporter human induced pluripotent stem cell (hiPSC) line using a CRISPR-Cas9-based knock-in approach in order to preserve function which could alter the biology of the cells. The reporter was found to faithfully track NKX2.5 expressing cells

SUBMITTER: Lopez-Muneta L 

PROVIDER: S-EPMC8815860 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature