Mechanistic Insight Into Cadmium- and Zinc-Induced Inactivation of the Candida albicans Pif1 Helicase.

ABSTRACT: Zinc and cadmium are environmental contaminants that can cause disease by affecting the activity of DNA-repair proteins. In this study, we investigated the effect of Zn²⁺ and Cd²⁺ on the Candida albicans Pif1, a DNA-repair helicase that plays a critical role in ensuring genomic stability. We show that Zn2+ and Cd2+ strongly inhibit both the ATPase and the unwinding activities of CaPif1, but have no effect on its DNA binding activity. High concentrations of Cd2+ may bind to the cysteine residues of CaPif1, and its inhibition appears to be difficult to be restored by ethylene diamine tetraacetic acid, while inhibition due to Zn²⁺ can. When the two ions are at low concentrations, increasing the concentration of ATP in the reaction can appropriately weak

SUBMITTER: Zhang B 

PROVIDER: S-EPMC8815974 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature