Correlative all-optical quantification of mass density and mechanics of subcellular compartments with fluorescence specificity.

ABSTRACT: Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution of viscoelastic properties inside biological samples - so far relying on the implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography and epifluorescence imaging for explicitly measuring the Brillouin shift, RI, and absolute density with specificity to fluorescently labeled structures. We show that neglecting the RI and density might lead to erroneous conclusions. Investigating the nucleoplasm of wild-type HeLa cells, we find that it has lower density but higher lo

SUBMITTER: Schlußler R 

PROVIDER: S-EPMC8816383 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature