Dataset Information

Forensics in motion - Historic vehicles, genuine or fake?

ABSTRACT: In-depth forensic investigation and analysis of historic vehicles is a relatively new field of research. However, such examinations are becoming an important tool in the face of rising market values and the associated increase of manipulations and forgeries. We review various forensic approaches used in our interdisciplinary work in this field, including vehicle specialists, metallurgists, restorers, and archaeometry laboratories. The related investigations cover a general examination of the vehicle including detailed analysis of its materials and construction techniques, which are cross-referenced with the techniques and materials used in period. We illustrate our theoretical and analytical approach with practical examples from our work on collector vehicles. Finally, we consider methods and approaches currently under development.

SUBMITTER: Tutt G

PROVIDER: S-EPMC8816669 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Forensics in motion - Historic vehicles, genuine or fake?

Tutt Gundula G Hoffmann Sebastian S

Forensic science international. Synergy 20220201

In-depth forensic investigation and analysis of historic vehicles is a relatively new field of research. However, such examinations are becoming an important tool in the face of rising market values and the associated increase of manipulations and forgeries. We review various forensic approaches used in our interdisciplinary work in this field, including vehicle specialists, metallurgists, restorers, and archaeometry laboratories. The related investigations cover a general examination of the veh ...[more]

PMID: 35141514

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Project description:BackgroundEstablishing reliable methods for assessing the microbiome within the built environment is critical for understanding the impact of biological exposures on human health. High-throughput DNA sequencing of dust samples provides valuable insights into the microbiome present in human-occupied spaces. However, the effect that different sampling methods have on the microbial community recovered from dust samples is not well understood across sample types. Heating, ventilation, and air conditioning (HVAC) filters hold promise as long-term, spatially integrated, high volume samplers to characterize the airborne microbiome in homes and other climate-controlled spaces. In this study, the effect that dust recovery method (i.e., cut and elution, swabbing, or vacuuming) has on the microbial community structure, membership, and repeatability inferred by Illumina sequencing was evaluated.ResultsThe results indicate that vacuum samples captured higher quantities of total, bacterial, and fungal DNA than swab or cut samples. Repeated swab and vacuum samples collected from the same filter were less variable than cut samples with respect to both quantitative DNA recovery and bacterial community structure. Vacuum samples captured substantially greater bacterial diversity than the other methods, whereas fungal diversity was similar across all three methods. Vacuum and swab samples of HVAC filter dust were repeatable and generally superior to cut samples. Nevertheless, the contribution of environmental and human sources to the bacterial and fungal communities recovered via each sampling method was generally consistent across the methods investigated.ConclusionsDust recovery methodologies have been shown to affect the recovery, repeatability, structure, and membership of microbial communities recovered from dust samples in the built environment. The results of this study are directly applicable to indoor microbiota studies utilizing the filter forensics approach. More broadly, this study provides a better understanding of the microbial community variability attributable to sampling methodology and helps inform interpretation of data collected from other types of dust samples collected from indoor environments.

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Forensics in motion - Historic vehicles, genuine or fake?

Publications

Forensics in motion - Historic vehicles, genuine or fake?

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