Spatial Organization and Prognostic Significance of NK and NKT-like Cells via Multimarker Analysis of the Colorectal Cancer Microenvironment.

ABSTRACT: Although tumor-infiltrating T cells hold a beneficial prognostic role in colorectal cancer, other lymphocytic populations are less characterized. We developed a multiplexed immunofluorescence assay coupled with digital image analysis and machine learning to identify natural killer (NK) cells (NCAM1⁺CD3^-), natural killer T-like (NKT-like) cells (NCAM1⁺CD3⁺), and T cells (NCAM1^-CD3⁺) within the PTPRC⁺ (CD45⁺) cell population and to measure their granzyme B (GZMB; cytotoxicity marker) and FCGR3A (CD16a; NK-cell maturity marker) expression. We evaluated immune cell densities and spatial configuration in 907 incident colorectal carcinoma cases within two prospective cohort studies. We found that T cells were

SUBMITTER: Vayrynen JP 

PROVIDER: S-EPMC8816895 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature