Reproductive stage- and season-dependent culture characteristics of enriched caprine male germline stem cells.

ABSTRACT: The present study aims to evaluate season- and reproductive-stage dependent variation in culture characteristics and expression of pluripotency and adhesion markers in caprine-male germline stem cells (cmGSCs). For this, testes from pre-pubertal (4-6 months) and adult (~ 2 years) bucks during non-breeding (July-August; n = 4 each) and breeding (October-November; n = 4 each) seasons were used to isolated testicular cells by two-step enzymatic digestion. After cmGSCs enrichment by multiple methods (differential platting, Percoll density gradient centrifugation, and MACS), cell viability of CD90⁺ cells was assessed before co-cultured onto the Sertoli cell feeder layer up to 3^rd-passage (P-3). The culture characteristics of cmGSCs were compared during primar

SUBMITTER: Singh SP 

PROVIDER: S-EPMC8816984 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature