Mouse characteristics that affect establishing xenografts from hepatocellular carcinoma patient biopsies in the United States.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) patient-derived xenograft (PDX) models hold potential to advance knowledge in HCC biology to help improve systemic therapies. Beside hepatitis B virus-associated tumors, HCC is poorly established in PDX.

Methods

PDX formation from fresh HCC biopsies were obtained and implanted intrahepatically or in subrenal capsule (SRC). Mouse liver injury was induced in immunodeficient Fah^-/-  mice through cycling off nitisinone after HCC biopsy implantation, versus continuous nitisinone as non-liver injury controls. Mice with macroscopically detectable PDX showed rising human alpha1-antitrypsin (hAAT) serum levels, and conversely, no PDX was observed in mice with undetectable hAAT.

Results

Using rising hAAT as a marker for PDX f

SUBMITTER: Zou C 

PROVIDER: S-EPMC8817074 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature