MiR-140-3p is a potential differential biomarker in benign phyllodes tumors and fibroadenoma of the breast.

ABSTRACT:

Background

Benign phyllodes tumor (BPT) and fibroadenoma (FA) have some difficulties in differential diagnosis. BPT is often misdiagnosed as FA during the first operation and is not diagnosed until postoperative recurrence and reoperation. The intent of this research was to find and validate microRNAs (miRNAs) with significant differential expression between BPT and FA as novel potential differential biomarkers.

Methods

Tissue specimens from three BPT patients and three FA patients were selected to detect the expression of miRNAs by miRNA-Seq technique. Primary cells were extracted and cultured from fresh BPT and FA tissues by tissue-block culture. The expression of differentially expressed miRNA (DEmiRNA) was further verified by quantitative real-time polymerase chain react

SUBMITTER: Li Q 

PROVIDER: S-EPMC8817532 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature