Chitosan induces differential transcript usage of chitosanase 3 encoding gene (csn3) in the biocontrol fungus Pochonia chlamydosporia 123.

ABSTRACT:

Background

Pochonia chlamydosporia is an endophytic fungus used for nematode biocontrol that employs its cellular and molecular machinery to degrade the nematode egg-shell. Chitosanases, among other enzymes, are involved in this process. In this study, we improve the genome sequence assembly of P. chlamydosporia 123, by utilizing long Pacific Biosciences (PacBio) sequence reads. Combining this improved genome assembly with previous RNA-seq data revealed alternative isoforms of a chitosanase in the presence of chitosan. This study could open new insights into understanding fungal resistance to chitosan and root-knot nematode (RKN) egg infection processes.

Results

The P. chlamydosporia 123 genome sequence assembly has been updated using long-read PacBio sequencing and now incl

SUBMITTER: Sambles C 

PROVIDER: S-EPMC8817618 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature