RNA m⁶A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation.

ABSTRACT: Although various strategies have been included in nanotoxicity evaluation, epitranscriptomics has rarely been integrated into this field. In this proof-of-concept study, N6-methyladenosine (m⁶A) changes of mRNA in HEK293T cells induced by three bovine serum albumin (BSA)-templated Au, CuS and Gd₂O₃ nanoparticles are systematically explored, and their possible biological mechanisms are preliminarily investigated. It has been found that all the three BSA-templated nanoparticles can reduce m⁶A levels, and the genes with reduced m⁶A are enriched for TGF-beta signaling, which is critical for cell proliferation, differentiation and apoptosis. Further results indicate that abnormal aggregation of m⁶A-related enzymes at least partly

SUBMITTER: Pan J 

PROVIDER: S-EPMC8817964 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature