TRNA modification profiles in obligate and moderate thermophilic bacilli.

ABSTRACT: Transfer RNAs (tRNAs) are the most ancient RNA molecules in the cell, modification pattern of which is linked to phylogeny. The aim of this study was to determine the tRNA modification profiles of obligate (Anoxybacillus, Geobacillus, Paragebacillus) and moderate (Bacillus, Brevibacillus, Ureibacillus, Paenibacillus) thermophilic aerobic bacilli strains to find out its linkage to phylogenetic variations between species. LC-MS was applied for the quantification of modified nucleosides using both natural and isotopically labeled standards. The presence of m²A and m⁷G modifications at high levels was determined in all species. Relatively high level of i⁶A and m⁵C modification was observed for Paenibacillus and Ureibacillus, respectively. The lowest

SUBMITTER: Panosyan H 

PROVIDER: S-EPMC8818000 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature