ScreenGarden: a shinyR application for fast and easy analysis of plate-based high-throughput screens.

ABSTRACT:

Background

Colony growth on solid media is a simple and effective measure for high-throughput genomic experiments such as yeast two-hybrid, synthetic dosage lethality and Synthetic Physical Interaction screens. The development of robotic pinning tools has facilitated the experimental design of these assays, and different imaging software can be used to automatically measure colony sizes on plates. However, comparison to control plates and statistical data analysis is often laborious and pinning issues or plate specific growth effects can lead to the detection of false-positive growth defects.

Results

We have developed ScreenGarden, a shinyR application, to enable easy, quick and robust data analysis of plate-based high throughput assays. The code allows comparisons of differ

SUBMITTER: Klemm C 

PROVIDER: S-EPMC8818250 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature