Comparison of S1 antibody titers between BNT162b2 and ChAdOx1 COVID-19 vaccination in cancer patients.
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ABSTRACT:
SUBMITTER: van Dam PA
PROVIDER: S-EPMC8818384 | biostudies-literature | 2022 Apr
REPOSITORIES: biostudies-literature
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Comparison of S1 antibody titers between BNT162b2 and ChAdOx1 COVID-19 vaccination in cancer patients.
van Dam P A PA Debie Y Y Teuwen L L Verbruggen L L Vanhoutte G G Peeters B B Croes L L Vulsteke C C Anguille S S Vandamme T T Peeters M M
ESMO open 20220207 2
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Project description:BackgroundChAdOx1 and BNT162b2 vaccines are currently commonly used against coronavirus disease 2019 worldwide. Our study was designed to determine the serostatus and relative levels of anti-S and neutralizing antibodies in patients who were administered either ChAdOx1 or BNT162b2 vaccine. In addition, we investigated whether the antibody response to each vaccine differed according to sex and age.MethodsHealthcare workers (HCWs) at a general hospital who were vaccinated with two doses of either ChAdOx1 or BNT162b2 were invited to participate in this prospective cohort study. Blood samples of HCWs vaccinated with both ChAdOx1 doses over a period of 12 weeks were collected at weeks 4 and 8 post first vaccination and 2 weeks post second vaccination. Blood samples of HCWs vaccinated with BNT162b2 were collected in the third week after the first dose, and the second dose was then administered on the same day; two weeks post second dose (5 weeks after the first dose), blood samples were collected to assess the antibody response. The titers of anti-S antibodies against the severe acute respiratory syndrome coronavirus 2 spike (S) protein receptor-binding domain and the neutralizing antibodies in the collected blood were evaluated.ResultsOf the 309 HCWs enrolled in the study, 205 received ChAdOx1 and 104 received BNT162b2. Blood samples from participants receiving either the ChAdOx1 or BNT162b2 vaccine exhibited substantial anti-S and neutralizing antibody seropositivity subsequent to the second dose. All participants (100%) from both vaccine groups were seropositive for anti-S antibody, while 98% (201/205) of ChAdOx1-vaccinated individuals and 100% (104/104) of BNT162b2-vaccinated individuals were seropositive for neutralizing antibodies. The median levels of anti-S and neutralizing antibodies were significantly higher in the BNT162b2-vaccinated group than the ChAdOx1-vaccinated group; in particular, anti-S antibody titers of 1,020 (interquartile range, 571.0-1,631.0) U/mL vs. 2,360 (1,243-2,500) U/mL, P < 0.05, were recorded for the ChAdOx1 and BNT162b2 groups, respectively, and neutralizing antibody titers of 85.0 (65.9-92.1%) vs. 95.8 (94.4-96.6%), P < 0.05, were recorded for the ChAdOx1 and BNT162b2 groups, respectively. In the ChAdOx1 vaccine group, the neutralizing antibody level was significantly higher in women than in men (85.7 [70.3-92.5%] vs. 77.7 [59.2-91.0%], P < 0.05); however, the neutralizing antibody titer in the BNT162b2 vaccine group did not vary between the two sexes (95.9 [95.2-96.6%] vs. 95.2 [93.5-96.3%], P = 0.200). Analysis of the correlation of antibody profiles with age revealed that the levels of anti-S antibodies and signal inhibition rate (SIR) of neutralizing antibodies decreased significantly with age.ConclusionBoth the ChAdOx1- and BNT162b2-vaccinated groups showed high seropositivity for anti-S and neutralizing antibodies. The SIR of neutralizing antibodies in the ChAdOx1 vaccine group was higher in women than in men. Enhanced antibody responses were observed in participants vaccinated with BNT162b2 compared to those vaccinated with the ChAdOx1 vaccine.
| S-EPMC8629719 | biostudies-literature
Project description:Introduction Le risque de thrombocytopénies thrombotiques immunitaires induites par le vaccin ChAdOx1-nCoV19 a conduit la France à recommander une seconde dose par un vaccin ARNm chez les personnes <55 ans ayant reçu une dose de ce vaccin. Nous avons évalué l'immunogénicité d'une vaccination hétérologue ChAdOx1-nCoV19/BNT162b2 et homologue BNT162b2/BNT162b2, et la réactogénicité dans le groupe ChAdOx1-nCoV19/BNT162b2. Matériels et méthodes Il s'agit d'une sous-étude nichée dans l'étude ANRS0001S COV-POPART (ClinicalTrials.gov NCT04824651), cohorte nationale française évaluant l'immunogénicité de la vaccination COVID-19 dans des populations particulières. Ont été inclus dans cette étude des participants âgés de 18 à 54 ans immunocompétents, sans antécédent de COVID-19: 1) groupe ChAdOx1-nCoV19/BNT162b2 (groupe 1) ayant reçu une dose de BNT162b2 12 semaines après une première dose de ChAdOx1-nCoV19; 2) groupe BNT162b2/BNT162b2 (groupe 2) ayant reçu 2 doses BNT162b2 à 28 jours d'intervalle. Les titres d'IgG anti-Spike SARS-CoV-2 (anti-S, ELISA) et d'IgG neutralisants spécifiques SARS-CoV-2 (test de neutralisation in vitro; souche Wuhan chez tous les sujets; Delta (B.1.617.2) et Omicron (B.1.1.529) dans un sous-échantillon), ont été évalués de façon centralisée 28 jours après la seconde dose de vaccin. La réactogénicité (événements indésirables (EI) locaux/systémiques sollicités) a été évaluée pendant 28 jours après la dose BNT162b2 dans le groupe 1. Résultats Au total, 180 participants ont été inclus dans le groupe 1 (69,4 % de femmes, âge médian 37 ans (IQR 29-44) et 227 dans le groupe 2 (50,4 % de femmes, âge de médian 44 ans (38-51)). Une séroconversion en IgG anti-S était observée chez 178 (98,9 %) participants du groupe 1 et tous les participants du groupe 2; les titres moyens géométriques (GMT) d'IgG anti-S étaient de 1 390,4 BAU/mL (IC95% 1262,8-1530,8) dans le groupe 1 et 1395,2 (1273,6-1528,3) dans le groupe 2; les titres d'IgG anti-S étaient >264 BAU/mL chez 176 (98,9%) et 220 (96,9%) participants des groupes 1 et 2 respectivement. Une séroconversion en anticorps neutralisants vis-à-vis de la souche Wuhan était mise en évidence chez 177 (99,4%) participants du groupe 1 et 223 (98,2%) du groupe 2, vis-à-vis du variant Delta chez 35/35 (100%) participants du groupe 1 et 10/10 (100%) participants du groupe 2, vis-à-vis du variant Omicron chez 32/35 (91,4%) participants du groupe 1 et 7/10 (70%) participants du groupe 2 (titres ≤ 40 chez 25/32 (78,1%) participants du groupe 1 et chez tous ceux du groupe 2). Des EI locaux ou systémiques (asthénie (74/106 (69,8%)), myalgies (47/106 (44,3%)), céphalées (52/107 (48,6)) ont été observés respectivement chez 123/157 (78,3%) et 107/160 (66,9%) participants du groupe 1. Conclusion Une vaccination hétérologue associant ChAdOx1-nCoV19 et BNT162b2 permet d'induire une réponse humorale robuste, un mois après la seconde injection avec un profil de réactogénicité acceptable. Aucun lien d'intérêt
| S-EPMC9152533 | biostudies-literature
Project description:Objectives: The BNT162b2 mRNA COVID-19 vaccine has been found to be highly effective in preventing COVID-19 but is associated with increased reactogenicity. We aimed to examine the correlation between immunogenicity and reactogenicity of the BNT162b2 vaccine. Methods: Subjects without prior SARS-CoV-2 infection that participated in active surveillance after being vaccinated with the BNT162b2 vaccine&nbsp;were included. Study participants reported adverse drug reactions (ADRs) through questionnaires administered by text message after receiving each dose of the vaccine. A reactogenicity score was developed based on the type and duration of ADRs. In addition, anti-receptor binding domain (RBD) levels and neutralization assays were performed 7-21 and 7-38 days after the first and second vaccine doses, respectively. Associations between ADRs and antibody levels were assessed by Spearman correlations. Multivariable logistic regression analyses were used to identify factors associated with ADRs. Results: A total of 831 health care workers were included. The mean age was 46.5 years (SD = 11.8) and 75.5% were females. 83.4% and 83.3% had at least one local ADR after the first and second doses, respectively. 33% and 83.2% had at least one systemic ADR after the first and second doses, respectively. Multivariate logistic regression analysis found a significant correlation between ADR score and anti-RBD-IgG titers (r = 0.366; p < 0.0001) after adjustment for age, gender, and days after the second vaccination. High anti-RBD-IgG levels, being younger than 55 and being female, were all correlated with increased rates of ADRs. Conclusion: BNT162b2 mRNA COVID-19 vaccine reactogenicity appears to be correlated with higher post-vaccination antibody levels and is independently associated with younger age and female gender.
| S-EPMC9412348 | biostudies-literature
Project description: Not available
| S-EPMC10496013 | biostudies-literature
Project description:Correlation between vaccine reactogenicity and immunogenicity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is unclear. Thus, we investigated to determine whether the reactogenicity after coronavirus disease 2019 vaccination is associated with antibody (Ab) titers and T cell responses. This study was prospective cohort study done with 131 healthcare workers at tertiary center in Seoul, South Korea. The degrees of the local reactions after the 1st and 2nd doses of ChAdOx1 nCov-19 (ChAdOx1) vaccination were significantly associated with the S1-specific IgG Ab titers (p=0.003 and 0.01, respectively) and neutralizing Ab (p=0.04 and 0.10, respectively) in age- and sex-adjusted multivariate analysis, whereas those after the BNT162b2 vaccination did not show significant associations. T cell responses did not show significant associations with the degree of reactogenicity after the ChAdOx1 vaccination or the BNT162b2 vaccination. Thus, high degree of local reactogenicity after the ChAdOx1 vaccine may be used as an indicator of strong humoral immune responses against SARS-CoV-2.
| S-EPMC8733188 | biostudies-literature
Project description:The COVID-19 pandemic is changing rapidly and requires different strategies to maintain immunization. In Korea, different COVID-19 vaccines are recommended and available for various populations, including healthcare workers (HCWs) at high risk of SARS-CoV-2 infection. We plan to evaluate the adverse events (AEs) and immunogenicity of the BNT162b2 and ChAdOx1 vaccines in HCWs at a single center. This cohort study included HCWs fully vaccinated with either BNT162b2 or ChAdOx1. Blood samples were taken eight weeks after the second vaccination with both COVID-19 vaccines and six months after the second vaccination from participants with the BNT162b2 vaccine. The primary endpoint for immunogenicity was the serum neutralizing antibody responses eight weeks after vaccination. The secondary endpoint was the incidence of various AEs within 28 days of each vaccination. Between 16 March and 23 June 2021, 115 participants were enrolled (65 in the ChAdOx1 group and 50 in the BNT162b2 group). Significantly higher surrogate virus neutralization test (sVNT) inhibition was observed in participants vaccinated with two doses of BNT162b2 (mean (SD) 91.4 (9.68)%) than in those vaccinated with ChAdOx1 (mean (SD) 73.3 (22.57)%). The effectiveness of the BNT162b2 vaccine was maintained across all age and gender categories. At six months after the second dose, serum antibody levels declined significantly in the BNT162b2 group. The main adverse events, including fever, myalgia, fatigue, and headache, were significantly higher in the ChAdOx1 group after the first dose, whereas, after the second dose, those AEs were significantly higher in the BNT162b2 group (p < 0.05). Two doses of either the ChAdOx1 or the BNT162b2 COVID-19 vaccine resulted in very high seropositivity among the HCWs at our center. The quality of the antibody response, measured by sVNT inhibition, was significantly better with the BNT162b2 vaccine than with the ChAdOx1 vaccine. There was no significant association between neutralizing antibody response and AE after each vaccination in our cohort.
| S-EPMC8708890 | biostudies-literature
Project description: Not available
| S-EPMC8321428 | biostudies-literature
Project description:Heterologous ChAdOx1-BNT162b2 vaccination induces a stronger immune response than BNT162b2-BNT162b2. Here we investigated the molecular transcriptome, germline allelic variants of immunoglobulin loci, and anti-Omicron antibody levels in 46 office and lab workers from the Republic of Korea following ChAdOx1-BNT162b2 vaccination. Anti-spike-specific IgG antibody levels against the ancestral SARS-CoV-2 strain increased from 70 AU/ml to 14,000 AU/ml to 142,000 AU/ml one, three and seven days following the second vaccination. Titers against VOC, including Omicron, were two- to three-fold lower, yet higher than those measured following BNT162b2-BNT162b2 vaccination. RNA-seq of peripheral immune cells demonstrated activation of interferon pathways with increased IGHV clonal transcripts encoding neutralizing antibodies. scRNA-seq revealed enriched B cell and CD4+ T cell responses in both ChAdOx1-BNT162b2 and BNT162b2-BNT162b2 recipients, but a stronger clonal expansion of memory B cells with ChAdOx1-BNT162b2. In summary, heterologous ChAdOx1-BNT162b2 provides an innate and adaptive immune response that exceeds homologous BNT162b2 vaccination.
2022-05-20 | GSE201535 | GEO
Project description: Not available
| S-EPMC8653144 | biostudies-literature
Project description:In several countries, thrombotic events after vaccination with ChAdOx1 nCoV-19 have led to heterologous messenger RNA (mRNA) boosting. We tested the antibody response to SARS-CoV-2 spike protein four weeks after heterologous priming with the ChAdOx1 (ChAd) vector vaccine followed by boosting with BNT162b2(ChAd/BNT), comparing data of homologous regimen (BNT/BNT, ChAd/ChAd) subjects positive for SARS-CoV-2 after the first dose of BNT162b2 (BNT1dose/CoV2) and convalescent COVID-19. healthy subjects naïve for SARS-CoV-2 infection were assessed for serum IgG anti-S-RBD response 21 days after priming (T1), 4 (TFULL) and 15 (T15W) weeks after booster dose. The median IgG anti-S-RBD levels at TFULL of Chad/BNT group were significantly higher than the BNT/BNT group and ChAd/ChAd. Those of BNT/BNT group were significantly higher than ChAd/ChAd. IgG anti-S-RBD of BNT1dose/CoV2 group were similar to BNT/BNT, ChAd/BNT and ChAd/Chad group. The levels among COVID-19 convalescents were significantly lower than ChAd/BNT, BNT/BNT, ChAd/Chad and BNT1dose/CoV2. The proportion of subjects reaching an anti-S-RBD titer >75 AU/mL, correlated with high neutralizing titer, was 94% in ChAd/BNT and BNT/BNT, 60% in BNT1dose/CoV2, 25% in ChAd/ChAd and 4.2% in convalescents. At T15W the titer of ChAd/BNT was still significantly higher than other vaccine schedules, while the anti-S-RBD decline was reduced for ChAd/ChAd and similar for other combinations. Our data highlight the magnitude of IgG anti-S-RBD response in ChAd/BNT dosing, supporting the current national guidelines for heterologous boosting.
| S-EPMC8704060 | biostudies-literature