CircRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.

ABSTRACT:

Background

Circular RNAs (circRNAs) represent a novel class of non-coding RNAs formed by a covalently closed loop and play crucial roles in many biological processes. Several circRNAs associated with myogenesis have been reported. However, the dynamic expression, function, and mechanism of circRNAs during myogenesis and skeletal muscle development are largely unknown.

Methods

Strand-specific RNA-sequencing (RNA-seq) and microarray datasets were used to profile the dynamic circRNAome landscape during skeletal muscle development and myogenic differentiation. Bioinformatics analyses were used to characterize the circRNAome and identify candidate circRNAs associated with myogenesis. Bulk and single-cell RNA-seq were performed to identify the downstream genes and pathways of circ

SUBMITTER: Yan J 

PROVIDER: S-EPMC8818660 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature