Visualization of Dynamic Mitochondrial Calcium Fluxes in Isolated Cardiomyocytes.

ABSTRACT:

Background

Cardiomyocyte contraction requires a constant supply of ATP, which varies depending on work rate. Maintaining ATP supply is particularly important during excitation-contraction coupling, where cytosolic Ca²⁺ fluxes drive repeated cycles of contraction and relaxation. Ca²⁺ is one of the key regulators of ATP production, and its uptake into the mitochondrial matrix occurs via the mitochondrial calcium uniporter. Fluorescent indicators are commonly used for detecting cytosolic Ca²⁺ changes. However, visualizing mitochondrial Ca²⁺ fluxes using similar methods is more difficult, as the fluorophore must be permeable to both the sarcolemma and the inner mitochondrial membrane. Our aim was therefore to optimize a method using th

SUBMITTER: Krstic AM 

PROVIDER: S-EPMC8818789 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature