Cytokines in the Immune Microenvironment Change the Glycosylation of IgG by Regulating Intracellular Glycosyltransferases.

ABSTRACT:

Background

Changes in IgG glycosylation, as a novel pathological feature, are observed in various autoimmune diseases (AIDs). The glycosylation patterns of IgG play a critical role in regulating the biological function and stability of IgG involved in the pathophysiology of many AIDs. However, the intracellular regulatory mechanisms underlying the effects of disturbances in various cytokines on IgG glycosylation are poorly understood. Thus, we investigated the regulatory effects of elevated cytokines in AIDs on intracellular IgG glycosylation within B cells.

Methods

First, we established a controlled primary culture system in vitro to differentiate human CD19⁺ B cells into antibody-secreting cells (ASCs). Then, the IgG concentrations in the supernatants wer

SUBMITTER: Cao Y 

PROVIDER: S-EPMC8818798 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature