Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption.

ABSTRACT: The number of man-made chemicals has increased exponentially recently, and exposure to some of them can induce fetal malformations. Because complex and precisely programmed signaling pathways play important roles in developmental processes, their disruption by external chemicals often triggers developmental toxicity. However, highly accurate and high-throughput screening assays for potential developmental toxicants are currently lacking. In this study, we propose a reporter assay that utilizes human-induced pluripotent stem cells (iPSCs) to detect changes in fibroblast growth factor signaling, which is essential for limb morphogenesis. The dynamics of this signaling after exposure to a chemical were integrated to estimate the degree of signaling disruption, which afforded a good prediction

SUBMITTER: Kanno S 

PROVIDER: S-EPMC8819105 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature