Relative and Quantitative Rhizosphere Microbiome Profiling Results in Distinct Abundance Patterns.

ABSTRACT: Next-generation sequencing is one of the most popular and cost-effective ways of characterizing microbiome in multiple samples. However, most of the currently available amplicon sequencing approaches are limited, as they result in relative abundance profiles of microbial taxa, which does not represent actual abundance in the environment. Here, we combined amplicon sequencing (16S rRNA gene for bacteria and ITS region for fungi) with real-time quantitative PCR (qPCR) to characterize the rhizosphere microbiome of wheat. We show that changes in the relative abundance of major microbial phyla do not necessarily follow the same pattern as the estimated quantitative abundance. Most of the bacterial phyla linked with the rhizosphere of plants grown in soil with no history of water stress showed e

SUBMITTER: Azarbad H 

PROVIDER: S-EPMC8819139 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature